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Study on characteristics of cellular-mediated immune responses of novel H1N1 influenza A patients with pneumonia / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology ; (6): 412-414, 2010.
Article in Chinese | WPRIM | ID: wpr-316886
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the phenotype, frequency and function of CD4+ T cell subsets and the relevant cytokines, as well as the relationship between these cells and appearance of pneumonia of novel (H1N1) influenza A patients.</p><p><b>METHODS</b>68 healthy people, 53 confirmed novel A(H1N1) influenza patients without pneumonia and 16 confirmed severe novel A (H1N1) influenza patients with pneumonia were enrolled in this study. Viral load in nasopharyngeal swabs specimens was measured by real time PCR assay. The phenotype and percentage of CD4+ T cell subsets including Th1, Th2, Th17, and Treg cells were measured by Flow cytometry analysis. The relevant cytokines in plasma including TGF-beta, IL-6 and IFN-gamma were measured by ELISA. Data was analyzed by one way ANOVA.</p><p><b>RESULTS</b>It was found that peak viral load and viral shedding period of severe patients with pneumonia was significantly increased compared with mild patients without pneumonia (P < 0.05). The percentage of Th17 cells of severe patients with pneumonia was significantly diminished compared to that of healthy subjects and mild patients without pneumonia (P < 0.05). However, Th1, Th2, Treg cells frequencies had no significant differences (P > 0.05) among these three groups. The level of TGF-beta in plasma for the severe patients with pneumonia was also significantly decreased compared to that of healthy subject and mild patients without pneumonia (P < 0.05). The viral shedding period inversely correlated with the frequency of Th17 cells (r = - 0.38, P < 0.05).</p><p><b>CONCLUSION</b>H1N1 influenza A virus can inhibit Th17 cells to differentiate, particularly more extent in patients with pneumonia. Impaired Th17 cells may correlate with viral clearance and pneumonia of novel H1N1 influenza A patients.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pneumonia, Viral / CD4-Positive T-Lymphocytes / Cytokines / T-Lymphocyte Subsets / Allergy and Immunology / Influenza, Human / Influenza A Virus, H1N1 Subtype / Immunity, Cellular Limits: Adolescent / Adult / Female / Humans / Male Language: Chinese Journal: Chinese Journal of Experimental and Clinical Virology Year: 2010 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pneumonia, Viral / CD4-Positive T-Lymphocytes / Cytokines / T-Lymphocyte Subsets / Allergy and Immunology / Influenza, Human / Influenza A Virus, H1N1 Subtype / Immunity, Cellular Limits: Adolescent / Adult / Female / Humans / Male Language: Chinese Journal: Chinese Journal of Experimental and Clinical Virology Year: 2010 Type: Article