Transmission electron microscopy investigation of the denudation of the envelope of influenza virus treated with Nonidet P-40 and the binding influence of antibody / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 340-342, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-316905
ABSTRACT
<p><b>OBJECTIVE</b>Through observing the morphological changes of the prepared influenza viruses (H1N1) treated with the different concentration of Nonidet P-40 solutions and added with antibody against the influenza virus using transmission electron microscopy (TEM), to explore the principle of application of the internal antibody for immunoassay of influenza virus.</p><p><b>METHODS</b>Through treating the virus samples with serial diluted Nonidet P-40 solutions from 0.01% to 0.2% and/or not adding antibody against the influenza virus, then investigating the samples by TEM to obtain the morphological changes of the virions.</p><p><b>RESULTS</b>The serial images show that the denudation degree of the virions is proportional with the rise of NP-40 concentration, and partly denuded virion image appeared at 0.1% NP-40 treatment, also under this condition no influence was observed on antibody binding to the virus.</p><p><b>CONCLUSION</b>This work demonstrated the interaction between influenza virion and its antibody under nonionic surfactants existing, which supports the advantages of sample preparation for immunoassay enveloped virus using internal antibody theoretically.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Polyethylene Glycols
/
Protein Binding
/
Virion
/
Chemistry
/
Viral Envelope Proteins
/
Microscopy, Electron, Transmission
/
Influenza A Virus, H1N1 Subtype
/
Antibodies, Viral
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2010
Type:
Article
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