Occult HBV infection in patients with anti-HBc positive alone / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 221-223, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-316917
ABSTRACT
<p><b>OBJECTIVE</b>This study was designed to explore the incidence rate of occult HBV infection in patients with anti-HBc positive alone and analyze the possible reasons of occult infection.</p><p><b>METHODS</b>Sera of 183 patients carrying anti-HBc alone(A < or = 0.1) were collected and real-time PCR was used to select samples with HBV DNA positive. HBV pre-S/S amplification products were obtained by PCR, and clonal sequencing were then used for these samples with HBV DNA positive.</p><p><b>RESULTS</b>DNA quantitative results of three samples were greater than 10(3) copies/ml in 183 samples, with a fraction of 1.6%. Pre-S/S sequencing results of two samples from these three samples were obtained. Point mutations within "a" determinant with Q129R/P mutations and co-existence of the mutant type and wild type were found in the two samples.</p><p><b>CONCLUSIONS</b>Occult HBV infection existed in samples with anti-HBc alone. Factors contributing to the loss of HBsAg detection by immunoassays include S gene mutations and low levels of circulating antigen which are below the assay limit of detection. Occult HBV infection not only can lead to a false clinical diagnosis, but also can result in hematological pollution due to such occult infection of blood donors.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Protein Precursors
/
Blood Donors
/
DNA, Viral
/
Base Sequence
/
Hepatitis B virus
/
Polymerase Chain Reaction
/
Diagnosis
/
Allergy and Immunology
/
Genetics
/
Genotype
Type of study:
Diagnostic study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2010
Type:
Article
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