Expression and purification of EBV-LMP2 protein / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To obtain a second Epstein-Barr virus membrane protein (LMP2) in insect cells.</p><p><b>METHODS</b>The full length EBV-LMP2 gene was inserted into baculovirus expression transfer vector pFastBac HT B to obtain the recombinant baculoviruses Bac-LMP2. And generation of recombinant baculoviruses was followed by transfection of the recombinant Bac-LMP2 into insect cells, then the recombinant LMP2 protein was recognized by SDS-PAGE and western blot. The expressed LMP2 protein was purified by one step with Ni-NTA metal chelation chromatography.</p><p><b>RESULTS</b>The expressed LMP2 protein was confirmed by SDS-PAGE and western blot. The purity of purified LMP2 protein is up to 86% by HPLC analysis.</p><p><b>CONCLUSION</b>The EBV-LMP2 was expressed in insect cells, and the purified LMP2 protein was obtained.</p>