Research of PD-1 expression in CD8+ T cell of peripheral blood with HBV-associated acute-on-chronic liver failure / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 125-127, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-316945
ABSTRACT
<p><b>OBJECTIVE</b>To analyze PD-1 expression in CD8 + T cell of Peripheral blood with HBV-associated acute-on-chronic liver failure and effect on CD8+ T cell.</p><p><b>METHODS</b>We selected 60 patients with HBV-ACLF and collected their peripheral blood. We analyzed the expressions of PD-1, CD95, perforin, granzyme A, granzyme B, CD107a on CD8+ T lymphocytes and the expression of PD-L1 on monocytes peripheral blood by using flow cytometry. 15 liver cirrhosis patients( LC) and 15 healthy individuals( HC) are control groups.</p><p><b>RESULT</b>PD-1 expression was (1) The PD-1 expression in HBV-ACLF patients was significantly elevated compared with those in HC and lower in improved group than that in invalid group and death group (P < 0.05) and increased from prophase, metaphase to advanced stage (P < 0.05). Moreover, (2) PD-L1 expression on monocytes was positively correlated with disease progression. (P < 0.05). (3) Both PD-1 and CD95 expressions were higher in dead group than those in improved and non-improved groups. Perforin, granzymes and CD107a expressions on CD8+ T cells significantly increased in dead group compared with those in improved and non-improved groups (P < 0.05). However, PD-1 expressions on these cells were lower, compared with normal persons.</p><p><b>CONCLUSIONS</b>The expression of PD-1 and PD-L1 in HBV-ACLF patients was positively correlated with disease progression. The elevated PD-1 expression promoted apoptosis of CD8+ T cells. For HBV-ACLF patients, the PD-1 expression on effector CD8+ T cells was lower than those in other CD8+ T cells, which maybe accounted for the failure to controlling immune injury in liver.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Virology
/
Virulence
/
Antigens, CD
/
Hepatitis B virus
/
Liver Failure, Acute
/
CD8-Positive T-Lymphocytes
/
Allergy and Immunology
/
Apoptosis Regulatory Proteins
/
Programmed Cell Death 1 Receptor
/
Flow Cytometry
Limits:
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2010
Type:
Article
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