Experimental research on anti-respiratory syncytial virus effect in vitro of earthworm coelomic fluid / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 116-118, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-316948
ABSTRACT
<p><b>OBJECTIVE</b>To explore the antivirus function in vitro of earthworm coelomic fluid (ECF) by researching its effect on inhibiting respiratory syncytial virus (RSV).</p><p><b>METHODS</b>By the method of Hep-2 cell culture and using ribavirin as a positive control, the anti-RSV effect of ECF was investigated by observing cytopathic effect (CPE) with MTT colorimetric assay.</p><p><b>RESULTS</b>In Hep-2 cells, the CC50 of ECF and ribavirin were 3.11 mg/ml and 1.35 mg/ml separately. In the experiment of ECF directly killing RSV, the IC50 of ECF was 184.1 microg/ml, SI was 16.87; In the experiment of ECF preventing RSV invasion, no antiviral function of ECF within the experimental concentration range was observed; In the experiment of ECF inhibiting RSV replication, the IC50 of ECF was 1555. 8 microg/ml, SI was 1.99.</p><p><b>CONCLUSION</b>ECF couldn't prevent virus from invading into host cell, but showed direct killing-virus function and inhibition of the virus replication.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Antiviral Agents
/
Oligochaeta
/
Pharmacology
/
Respiratory Syncytial Viruses
/
Virus Replication
/
Cell Line
/
Chemistry
/
Inhibitory Concentration 50
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2010
Type:
Article
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