Transient expression and identification of gene P and NP of NDV LaSota strain in two different cells / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 62-64, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-316964
ABSTRACT
<p><b>OBJECTIVE</b>To Construction of P and NP genes eukaryotic expression vectors of Newcastle Disease Virus LaSota strain,study its reverse genetics and functional genome of NDV.</p><p><b>METHODS</b>P, NP genes were amplified and cloned into pGEM-T easy vector and then subcloned into pcDNA3.1 (+) expression vector respectively, the recombinant plasmids were named pcDNA3.1 (+)-P and pcDNA3.1 (+)-NP, Recombinant plasmids were transfected into 293 and BHK-21 cells respectively and were detected using IE and Western blot analysis.</p><p><b>RESULTS</b>Expression of P, NP genes were detected and confirmed by the IE and WB analysis.</p><p><b>CONCLUSION</b>The recombinant eukaryotic plasmids pcDNA3. 1(+)-P, pcDNA3.1 (+)-NP were expressed in 293 and BHK-21 cells successfully. This research may be helpful for further study of reverse genetics and functional genome of NDV.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phosphoproteins
/
Viral Proteins
/
Newcastle disease virus
/
Gene Expression
/
Cell Line
/
Genetic Vectors
/
Genetics
/
Metabolism
/
Nucleoproteins
Type of study:
Diagnostic study
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2010
Type:
Article
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