Construction and identification of a vector inserted with complete genome of poliovirus strain Sabin I / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 59-61, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-316965
ABSTRACT
<p><b>OBJECTIVE</b>To develop a vector inserted with complete genome of poliovirus strain Sabin I.</p><p><b>METHODS</b>The 3 fragments of the complete genome of Sabin I was amplified and cloned to pEASY-T3 by molecular biological technology. These cloned pEASY-T3 were then digested by Restriction enzymes and ligated to pWSK29 step by step and identified.</p><p><b>RESULTS</b>The complete genome of poliovirus strain Sabin I was successfully cloned into vector pWSK29 with 9 nucleotide mutations.</p><p><b>CONCLUSION</b>The complete genome plasmid was constructed and it provided a basis for further research of the function of Sabin I.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Genome, Viral
/
Cloning, Molecular
/
Poliovirus
/
Genetic Vectors
/
Genetics
/
Mutation
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2010
Type:
Article
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