Effects of Ayurvedic Rasayana botanicals on CYP3A4 isoenzyme system / 中西医结合学报
Journal of Integrative Medicine
;
(12): 165-172, 2015.
Article
in English
| WPRIM
| ID: wpr-317091
ABSTRACT
<p><b>OBJECTIVE</b>Consuming botanical dietary supplements or herbal drugs along with prescription drugs may lead to potential pharmacokinetic-pharmacodynamic (PK-PD) herb-drug interactions (HDI). The present study focuses on the importance of and novel approach for assessing HDI in integrative medicine with case examples of two frequently-used Ayurvedic Rasayana botanicals.</p><p><b>METHODS</b>The aqueous extracts of Asparagus racemosus (ARE) and Gymnema sylvester (GSE) were prepared as per Ayurvedic Pharmacopoeia of India. Chemoprofiling of these extracts was done using high-performance liquid chromatography (HPLC). Additionally, ARE was characterized for the presence of shatavarins IV and I using HPLC & mass spectroscopy respectively. Effects of ARE and GSE were investigated on rat liver microsome using testosterone probe drug assay. The changes in formation of metabolite (6-β hydroxy testosterone) were monitored on incubation of testosterone alone, testosterone with ketoconazole, ARE and GSE using HPLC. Half inhibitory concentration (IC50) was used to predict plausible HDI.</p><p><b>RESULTS</b>ARE and GSE showed no inhibition with IC50 values >1 000 μg/mL while the standard inhibitor ketoconazole completely abolished CYP3A4-dependent activity at 0.531 μg/mL and IC50 was found to be 0.036 μg/mL.</p><p><b>CONCLUSION</b>ARE and GSE prepared as per Ayurvedic Pharmacopoeia of India were found to be safe for CYP3A4-mediated inhibitory HDI in rats. Our in vitro study suggests the need of further in vivo investigation for HDI in order to provide clinical relevance.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Plant Extracts
/
Chromatography, High Pressure Liquid
/
Rats, Wistar
/
Asparagus Plant
/
Gymnema sylvestre
/
Herb-Drug Interactions
/
Cytochrome P-450 CYP3A
/
Cytochrome P-450 CYP3A Inhibitors
/
Isoenzymes
Limits:
Animals
Language:
English
Journal:
Journal of Integrative Medicine
Year:
2015
Type:
Article
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