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Experiment on induction of fibroblasts on 3-D cell-foam structures to express osteoblastic phenotype and its mechanism / 中华外科杂志
Chinese Journal of Surgery ; (12): 271-274, 2006.
Article in Chinese | WPRIM | ID: wpr-317167
ABSTRACT
<p><b>OBJECTIVE</b>To study the feasibility of osteogenic phenotype expression by human skin fibroblasts induced in polyglycolic acid (PGA) foams and the effect of tumor necrosis factor-alpha (TNF-alpha) on the expression of bone morphogenetic protein (BMP) receptors.</p><p><b>METHODS</b>The fibroblasts were isolated, purified from human skin. (1) Fibroblasts were seeded onto PGA foams. The cell-PGA complexes were cultured in RCCS for 6 weeks, in the media of TNF-alpha (50 U/ml) and BMP-2 (0.1 microg/ml). 1 d, 3 and 6 weeks later, cells and extracellular matrix were investigated by electron microscopic and histochemistry observation respectively. Secretion of osteogenic markers were analyzed by biochemical methods. (2) Fibroblasts were seeded on the glass fragments or culture flasks and treated with TNF-alpha (50 U/ml) in different usage (one-time, all-time). The RT-PCR method and the immunohistochemistry fluorescence staining were used to examine the influence of TNF-alpha on the mRNA expression and the protein expression of the type I BMP receptors at 2, 4, 6, 8 d after treatment.</p><p><b>RESULTS</b>Fibroblasts seeded on the PGA foams formed 3-dimensional matrix 3 weeks after seeding, which was demonstrated as osteo-tissue by tetracycline labeling and ARS staining. Cells secreted much more bone-specific alkaline phosphatase (B-AKP) and osteocalcin (OCN) into supernatant than the cells that were cultured in the media without TNF-a and BMP2. Eight days after all-time usage, the TNF-alpha (50 U/ml) increased the expression of the mRNA and protein of the type IB BMP receptor.</p><p><b>CONCLUSIONS</b>Fibroblasts on 3-D cell-foam structures can express osteoblastic phenotype under certain inducing conditions. The numerous fibroblasts in body would be a promising resource for cell seeds candidate of tissue- engineered bone. TNF-alpha provides the essential condition for BMP2's target effect on fibroblasts, and combined use of TNF-alpha and BMP2 is one of the regulating factors.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Phenotype / Polyglycolic Acid / Cell Differentiation / Cells, Cultured / Transforming Growth Factor beta / Tumor Necrosis Factor-alpha / Cell Culture Techniques / Bone Morphogenetic Proteins / Cell Biology Limits: Humans Language: Chinese Journal: Chinese Journal of Surgery Year: 2006 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Phenotype / Polyglycolic Acid / Cell Differentiation / Cells, Cultured / Transforming Growth Factor beta / Tumor Necrosis Factor-alpha / Cell Culture Techniques / Bone Morphogenetic Proteins / Cell Biology Limits: Humans Language: Chinese Journal: Chinese Journal of Surgery Year: 2006 Type: Article