Role of serum from asphyxiated neonates in the inducement of human renal tubular cell adhesion to neutrophils / 中国当代儿科杂志
Chinese Journal of Contemporary Pediatrics
;
(12): 633-636, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-317373
ABSTRACT
<p><b>OBJECTIVE</b>To study the role of serum from asphyxiated neonates in the inducement of human renal proximal tubular epithelial cells (HK-2) adhesion to neutrophils and possible mechanisms.</p><p><b>METHODS</b>HK-2 cells were cultured randomly with 20% serum from neonates (1, 3, and 7 days after asphyxia), pyrrolidine dithiocarbamate (PDTC) or placebo. The activity of myeloperoxidase (MPO), an indicator of adhesion ability of HK-2 cells to neutrophils in suspensions, was detected by the biochemistry assay. Intercellular adhesion molecule-1 (ICAM-1) and nuclear factor-kappaB (NF-kappaB) of HK-2 cells were examined with the immunohistochemical staining.</p><p><b>RESULTS</b>The expression of MPO in the post-asphyxial 1-day serum treatment group were significantly higher than that in the PDTC treatment and the control groups as well as the post-asphyxial 3 and 7-day serum treatment groups (P<0.01). The expression of ICAM-1 and NF-kappaB in the post-asphyxial 1-day serum treatment group was also significantly higher than that in the other groups (P<0.01).</p><p><b>CONCLUSIONS</b>Serum from asphyxiated neonates can induce HK-2 cell adhesion to neutrophils, possibly through activating NF-kappaB and increasing the synthesis and expression of ICAM-1 on the surface of renal tubular epithelial cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Physiology
/
Asphyxia Neonatorum
/
Blood
/
Cell Adhesion
/
Cells, Cultured
/
NF-kappa B
/
Intercellular Adhesion Molecule-1
/
Kidney Tubules
/
Metabolism
Type of study:
Controlled clinical trial
Limits:
Humans
/
Infant, Newborn
Language:
Chinese
Journal:
Chinese Journal of Contemporary Pediatrics
Year:
2008
Type:
Article
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