HER-2/neu Oncogene Amplification by Chromogenic in situ Hybridization and Immunohistochemical Expression of Topoisomerase II-alpha in the Breast Cancer
Korean Journal of Pathology
;
: 26-34, 2003.
Article
in Korean
| WPRIM
| ID: wpr-31749
ABSTRACT
BACKGROUND:
Amplifications of the HER-2/neu oncogene and the Topoisomerase II-alpha gene are important determiners of the response to chemotherapy in the breast cancer. For detecting HER-2/neu amplification, fluorescent in situ hybridization and immunohistochemistry are currently regarded as standard methods. Chromogenic in situ hybridization (CISH) is investigated as a new modification of in situ hybridization. The purpose of this study is to compare the efficacy of CISH and immunohistochemistry (IHC) in detecting HER-2/neu oncogene amplification and to investigate the prognostic significance of the HER-2/neu oncogene and the Topoisomerase II-alpha gene in breast cancer.METHODS:
Using CISH and IHC the amplifications and protein expressions of the HER-2/neu oncogene were studied on paraffin sections of 43 infiltrating duct carcinomas. The expression of the Topoisomerase II-alpha gene was studied immunohistochemically.RESULTS:
Of the 43 infiltrating duct carcinomas, amplifications of the HER-2/neu oncogene by CISH were observed in 8 cases (18.6%), and the HER-2/neu protein was deemed overexpressed by IHC in 9 cases (20.9%). The amplifications of the HER-2/neu oncogene showed a statistically significant correlation with tumor size, histological grade, and the Topoisomerase II-alpha index. The Topoisomerase II-alpha index showed a statistically significant correlation with tumor size, lymph node status, stage, histologic grade, and estrogen receptor status.CONCLUSIONS:
CISH is a useful alternative for determining HER-2/neu amplification, especially for confirming the immunohistochemical staining results. HER-2/neu amplification and the Topoisomerase II-alpha gene index may be prognostic factors of breast cancer.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oncogenes
/
Paraffin
/
Breast
/
Breast Neoplasms
/
Immunohistochemistry
/
Mammary Neoplasms, Animal
/
In Situ Hybridization, Fluorescence
/
In Situ Hybridization
/
Drug Therapy
/
Estrogens
Type of study:
Prognostic study
Limits:
Animals
Language:
Korean
Journal:
Korean Journal of Pathology
Year:
2003
Type:
Article
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