Effects of different pH conditions on ffh gene expression in Streptococcus mutans / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 23-26, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-317737
ABSTRACT
<p><b>OBJECTIVE</b>This research aimed to detect the expression levels of ffh gene in Streptococcus mutans (S. mutans) UA159 under different pH conditions, analyze the effect of pH on the expression of ffh gene in S. mutans, and identify the factors regulating the ffh gene expression.</p><p><b>METHODS</b>Samples of S. mutans were collected at different growth stages (4 h, 18 h) and pH values (pH 4.0-7.0). Fluorescence quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure the relative mRNA expression and trend of the target gene ffh in S. mutans at different growth stages and pH values.</p><p><b>RESULTS</b>qRT-PCR results showed that the ffh gene expression decreased along with pH at 4 h, but the expression increased with decreasing pH at 18 h. Under the same pH conditions, the ffh gene expression was significantly different between 4 h and 18 h (P < 0.05).</p><p><b>CONCLUSION</b>Growth stage and pH value influenced the ffh gene expression in S. mutans.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Streptococcus mutans
/
Bacterial Proteins
/
Gene Expression
/
Gene Expression Regulation, Bacterial
/
Hydrogen-Ion Concentration
Type of study:
Prognostic study
Language:
Chinese
Journal:
West China Journal of Stomatology
Year:
2016
Type:
Article
Similar
MEDLINE
...
LILACS
LIS