Establishment of localization ultrathin section for cytopathic cells / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 376-378, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-318015
ABSTRACT
<p><b>OBJECTIVE</b>To establish a localization ultrathin section method through which target cytopathic cells could be sectioned in situ.</p><p><b>METHODS</b>Lab-Tek Chamber slide system (177402) was selected as resin embedding mould. Cells infected with Human adenovirus type 5 (Ad5) or A/HN/SWL3/ 2009 (H1N1) influenza virus were embedded in situ as models. Target cytopathic cells were exposed by trimming, sectioned and observed under transmission electron microscope (TEM).</p><p><b>RESULTS</b>Target cells could be sectioned in situ and virus particles could be found easily on sections.</p><p><b>CONCLUSION</b>A localization ultrathin sectioning method was established and this technique could be applied in virus detection in cytopathic cells to improve TEM detection efficiency.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Physiology
/
Virology
/
Cell Line
/
Adenovirus Infections, Human
/
Adenoviruses, Human
/
Microscopy, Electron, Transmission
/
Influenza, Human
/
Influenza A Virus, H1N1 Subtype
/
Methods
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2013
Type:
Article
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