Expression of TGF-beta1/Smad protein in rat liver fibrosis model and the role of IFN-gamma / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 340-343, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-318026
ABSTRACT
<p><b>OBJECTIVE</b>To study the impact of IFN-gamma on liver fibrosis and its possible mechanism. Thirty healthy male SD rats were randomly divided into two groups fibrosis model group, IFN-gamma treatment group. Experimental liver fibrosis was induced by subcutaneous injection of CCl4. After 12-week-treatment, serum hyalurnic acid and TGF-beta1 was examined, histopathological changes and degrees of fibrosis were observed by optical microscopy. Meanwhile, the expression of TGF-beta1, TbetaR- I and Smad2/3 proteins was detected by immunohistochemistry and quantified by using computerized image analysis.</p><p><b>RESULTS</b>(1) Pathological observation of hepatic specimens histological examination showed that there were significant difference between normal group and fibrosis model group by comparing with the degrees of inflammation and fibrosis (P < 0.05). And the difference between fibrosis model group and IFN-gamma treatment group was significant (P < 0.05). (2) Changes of the hepatic fibrosis index (serum HA and TGF-beta1) the levels of serum HA, TGF-beta1 in fibrosis model group were higher than IFN-gamma treatment groups (P < 0.05). (3) Changes of gene protein levels about TGF-beta1/Smad the expressions of TGF-beta1, TbetaR- I and Smad2/3 in rat hepatic tissue were detected with immunohistochemistry techniques. The expressions of the three items in model group were higher than normal group (P < 0.01). The difference between model group and IFN-gamma treatment group was significant (P < 0.05);</p><p><b>CONCLUSION</b>IFN-gamma treatment group had significant results on treating experimental hepatic fibrosis. By the way of inhibiting expressions of TGF-beta1, TbetaR- I, Smad2/3, IFN-gamma treatment group exerted its anti-fibrosis effect.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Interferon-gamma
/
Rats, Sprague-Dawley
/
Therapeutic Uses
/
Disease Models, Animal
/
Drug Therapy
/
Smad2 Protein
/
Smad3 Protein
/
Transforming Growth Factor beta1
/
Genetics
/
Liver
Type of study:
Prognostic study
Limits:
Animals
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2013
Type:
Article
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