Construction and characterization of EGFP reporter gene labeled Sindbis virus / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To construct and characterize EGFP reporter gene labeled Sindbis virus (SINV).</p><p><b>METHODS</b>The reporter gene EGFP was inserted into the genome of infectious clone pBR-XJ160 by using multi-fusion long fragment PCR method. Then apply reverse genetic manipulation technique to rescue and obtain EGFP labeled SINV.</p><p><b>RESULTS</b>We successively obtained labeled SINV, which has good fluorescent expression characteristics and genetic stability.</p><p><b>CONCLUSION</b>The labeled virus can be seen in living cells and living body, and this serves as a good tool for cell and tissue tropism and biological function study of viruses. This study laid a foundation for further studying the cell tropism, biological functions and infection mechanism of SINV.</p>