A novel SELEX method for screening of HIV-1 P24 antigen / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 218-220, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-318059
ABSTRACT
<p><b>OBJECTIVE</b>To screen aptamers that can bind P24 antigen tightly and specificly, and verify its specificity and affinity.</p><p><b>METHODS</b>Polycarbonate PCR plate was coated with P24 antigen and SELEX technology was used to screen aptamer on the PCR plate. The primary and secondary structure of these aptamers was analyzed by software. Through HRP labeled streptavidin and biotin labeled aptamers, the affinity and specificity of obtained aptamers were verified by ELISA.</p><p><b>RESULTS</b>The polycarbonate PCR plate could be coated with P24 antigen. Electrophoretic analysis showed the aptamers had been enriched. Sequence aligment analysis showed that these aptamers have consensus sequence and their apatial structure was multiple; ELISA verified that aptamers had high affinity with P24 antigen.</p><p><b>CONCLUSION</b>A simple method was established for screening aptamers that can bind HIV-1 P24 antigen specificly and tightly.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Polymerase Chain Reaction
/
HIV-1
/
HIV Core Protein p24
/
Allergy and Immunology
/
SELEX Aptamer Technique
/
Methods
Type of study:
Diagnostic study
/
Screening study
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2013
Type:
Article
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