Reverse genetics platform construction of influenza pandemic virus strain / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
;
(6): 85-88, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-318097
ABSTRACT
<p><b>OBJECTIVE</b>Reverse genetics was used to construct the platform of flu pandemic strain A/California/07/2009 (H1N1).</p><p><b>METHODS</b>Eight genes fragements were amplified and ligated with bidirectional vector, recombinant plasmids were co transfected to the 293 T cells and rescued the virus. Gene sequencing, antigenic analysis and growth property were used to evaluate the rescued virus.</p><p><b>RESULTS</b>Rescued virus show the genes sequence correct, keep the same antigenicity and similar growth property compared with wild type virus.</p><p><b>CONCLUSION</b>The pandemic virus reverse genetics platform of A/California/07/2009 (H1N1) were built. Based on this platform, rescued virus hold the similarity of antigenicity and growth ability with wild type virus.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Orthomyxoviridae
/
Plasmids
/
Allergy and Immunology
/
Pandemics
/
Reverse Genetics
/
Genetics
Language:
Chinese
Journal:
Chinese Journal of Experimental and Clinical Virology
Year:
2013
Type:
Article
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