Transfection of pEGFP-C2 in brain mediated by targeting liposome P-MMA-DOSPER / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 1170-1174, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-318191
ABSTRACT
This research tried improving the specificity and efficiency of gene transfection in gene therapy and tried making the liposome a better gene transfer vector to brain by use of the monoclonal antibody (anti-Lex/SSEA-1)-mediated targeting of liposome. The derivatized monoclonal antibody was conjugated to the liposome DOSPER to form the targeting liposome P-MMA-DOSPER. Then, the pEGFP-C2 encapsulated in P-MMA-DOSPER or DOSPER was injected into the lateral ventricle of SD rats respectively, and the brains were taken for frosted slice 1, 3, 7 or 14 days later. The expression of GFP was observed under fluorescent microscope. There was a lot of expression of GFP around the lateral ventricle of rats in each group. But the indirect fluorescence antibody test showed the ratio of GFP+/nestin+ cells to nestin+ cells of every marking time point in the group of P-MMA-DOSPER was higher than the one in the group of DOSPER; the difference was found to be statistically significant (P<0.01). The results proved that the P-MMA-DOSPER can permeat the ependyma and can transfer gene into the nerve stem cells in vivo safely and effectively.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Brain
/
Fatty Acids, Monounsaturated
/
Transfection
/
Rats, Sprague-Dawley
/
Gene Transfer Techniques
/
Polymethyl Methacrylate
/
Green Fluorescent Proteins
/
Genetic Vectors
/
Genetics
/
Liposomes
Limits:
Animals
Language:
Chinese
Journal:
Journal of Biomedical Engineering
Year:
2008
Type:
Article
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