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Determination of the activity of semicarbazide-sensitive amine oxidase in the differentiated 3T3-F442A cells by enzyme-linked spectrophotometric assay / 生物医学工程学杂志
Journal of Biomedical Engineering ; (6): 1161-1165, 2008.
Article in Chinese | WPRIM | ID: wpr-318193
ABSTRACT
In order to probe into the patho-physiology semicarbazide-sensitive amine oxidase (SSAO), we determined the variation of its activity in the days of 3T3-F442A cells' differentiation into adipocytes in vitro. The differentiated 3T3-F442A cells were collected in various days and disrupted by ultrasonication. Then we studied the SSAO activity of the collected cells by oxidase-linked spectrophotometric assay. In the course of 3T3-F442A cells' differentiation, SSAO expression was shown by a curve graph. There was no SSAO detected in 3T3-F442A preadipocyte, and only a little of SSAO was detected in the cells on the 3rd day of differentiation. Since then, SSAO significantly increased and reached to a maximum level six or seven days after the cells' differentiation.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Physiology / Spectrophotometry / Cell Differentiation / 3T3 Cells / Amine Oxidase (Copper-Containing) / Adipocytes / Cell Biology / Metabolism / Methods Type of study: Diagnostic study Limits: Animals Language: Chinese Journal: Journal of Biomedical Engineering Year: 2008 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Physiology / Spectrophotometry / Cell Differentiation / 3T3 Cells / Amine Oxidase (Copper-Containing) / Adipocytes / Cell Biology / Metabolism / Methods Type of study: Diagnostic study Limits: Animals Language: Chinese Journal: Journal of Biomedical Engineering Year: 2008 Type: Article