Pulsed-field gel electrophoresis typing on non-O157 Shiga toxin-producing Escherichia coli isolates / 中华流行病学杂志
Chinese Journal of Epidemiology
;
(12): 503-506, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-318366
ABSTRACT
<p><b>OBJECTIVE</b>To establish a database and to understand the molecular epidemiological features of non-O157 Shiga toxin-producing Escherichia coli (STEC) isolates from different animal reservoirs and patients.</p><p><b>METHODS</b>Pulsed-field gel electrophoresis (PFGE) was performed according to the PulseNet protocol with minor modifications. A dendrogram was constructed using the BioNumerics.</p><p><b>RESULTS</b>Under the PulseNet protocol, 62 PFGE patterns were obtained from 76 non-O157 STEC isolates and then divided into A to M groups. Isolates from different sources were widely distributed in different groups, but were predominant seen in certain groups.</p><p><b>CONCLUSION</b>The non-O157 STEC isolates in China were highly polymorphic. PulseNet protocol seemed to be suitable for the typing of Chinese non-O157 STEC isolates.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
DNA, Bacterial
/
China
/
Epidemiology
/
Electrophoresis, Gel, Pulsed-Field
/
Escherichia coli O157
/
Escherichia coli Infections
/
Shiga-Toxigenic Escherichia coli
/
Feces
/
Genetics
/
Genotype
Limits:
Animals
/
Humans
Country/Region as subject:
Asia
Language:
Chinese
Journal:
Chinese Journal of Epidemiology
Year:
2013
Type:
Article
Similar
MEDLINE
...
LILACS
LIS