Rapid recognition and identification of chemical constituents in Qinghuo Zhimai tablets by SPE-HPLC-ESI-MS(n) / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 186-192, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-318695
ABSTRACT
<p><b>OBJECTIVE</b>To establish a method for analyzing solid phase extraction-high performance liquid chromatography-electrospray ionization tandem mass spectrometry (SPE-HPLC-ESI-MS(n)), in order to recognize and identify the main chemical constituents in Qinghuo Zhimai tablets.</p><p><b>METHOD</b>The possible structures of the compounds were determined according to the structure information of compounds observed from molecular ion peaks and fragment ions in HPLC-ESI-MS(n) negative ion mode and by comparing with literature data or control samples.</p><p><b>RESULT</b>Through the comparative analysis on Qinghuo Zhimai Tablets and components of its formula, 39 chemical constituents were identified, including 7 caffeoylquinic acids, 7 iridoids, 6 diterpenoid lactones, 5 homoisoflavonoids, 13 steroidal saponins and 1 flavone glycoside.</p><p><b>CONCLUSION</b>This study provides a simple and rapid method for identifying chemical components in Qinghuo Zhimai tablets.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Quinic Acid
/
Saponins
/
Tablets
/
Drugs, Chinese Herbal
/
Molecular Structure
/
Chemistry
/
Chromatography, High Pressure Liquid
/
Spectrometry, Mass, Electrospray Ionization
/
Iridoids
/
Flavones
Type of study:
Diagnostic study
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2013
Type:
Article
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