Change in dissolution of chemical components of frankincense-myrrh before and after their compatibility and effect on no release of LPS-induced macrophage cells / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 179-185, 2013.
Article
in Zh
| WPRIM
| ID: wpr-318696
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To analyze the difference of chemical compounds of frankincense-myrrh before and after their compatibility, and evaluate the effect of differentiated compounds on NO generated by LPS-induced peritoneal macrophage cells in rats, in order to discuss synergetic material basis of frankincense-myrrh compatibility from the prospective of change in chemical constituents.</p><p><b>METHOD</b>UPLC-Q-TOF-MS/MS combined technology was used to analyze the chemical components of frankincense-myrrh before and after their compatibility. MarkerLynx 4. 1 statistical software was used to analyze differentiated compounds before and after their compatibility.</p><p><b>RESULT</b>The results of PCA showed that there were significant differences in the combined extracts of frankincense-myrrh and the chromatogram of their combined liquid, suggesting significant differences in their chemical compounds before and after their compatibility; after their compatibility, the dissolution of pentacyclic triterpenoid (alpha-boswellic acid, beta-boswellic acid) and tetracyclic triterpenoid (elemonic acid, 3-acetoxy-16-hydroxy-dammar-24-ene, 3-hydroxytirucalla-8,24-dien-21-oic acid or 3-hydroxytirucalla-7,24-dien-21-oic acid) increased notably, while the dissolution of both yclic sesquiterpenes and macrocyclic diterpenoids decreased. According to the evaluation on in vitro activity, 2-methoxy-8, 12-epoxy-germa-1 (10), 7, 11-triene-6-ketone, 2-methoxy-5-acetoxyl-furan-germa-1 (10)-alkene-6-ketone and 3-carbonyl Euphorbia kansui-8, 24-diene-21-carboxylic acid notably inhibited NO generated by LPS-induced peritoneal macrophage cells in rats.</p><p><b>CONCLUSION</b>These findings provide scientific basis and reference for studies on anti-inflammatory material basis of frankincense-myrrh compatibility.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Terpenes
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Triterpenes
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Drugs, Chinese Herbal
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Molecular Structure
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Chemistry
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Anti-Inflammatory Agents, Non-Steroidal
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Lipopolysaccharides
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Chromatography, Liquid
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Macrophages, Peritoneal
Limits:
Animals
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2013
Type:
Article