Effect of tetrandrine, toremifene and their combination on the reversion of multidrug resistance of K562/A02 cell line / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 61-64, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-318716
ABSTRACT
This study was aimed to investigate the reversible effect of tetrandrine, toremifene and their combination on multidrug resistance of K562/A02 cell line. The IC(50) (the concentration causing 50% inhibition of cell growth) of adriamycin (ADR) were assayed by MTT method, the expression of MDR1 mRNA was measured by RT-PCR, the concentration of p-glycoprotein (P-gp) and intracellular ADR were detected by flow cytometry. The results showed that the IC(50) of ADR on K562/A02 and K562 cells were 57.43 and 1.16 mg/L, respectively. The IC(50) of ADR on K562/A02 cells after treatment with tetrandrine, toremifene and both combination were 14.12, 20.74 and 9.14 mg/L respectively, but both drugs did not influence the IC(50) of ADR on K562 cells. Pretreating K562/A02 cells with toremifene (2.5 micromol/L), tetrandrine (1 micromol/L) or both for 72 hours partially restored the sensitivity of K562/A02 cells to ADR. Tetrandrine and toremifene (alone or combination) elevated the ADR concentration in K562/A02, down regulated the expressions of P-gp and MDR1 mRNA. It is concluded that multidrug resistance of K562/A02 cells can be partially reversed by tetrandrine or toremifene, the combination of both drugs shows a higher synergistic reversal effect.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Doxorubicin
/
Toremifene
/
Drug Resistance, Multiple
/
Drug Resistance, Neoplasm
/
Antineoplastic Agents, Hormonal
/
K562 Cells
/
Benzylisoquinolines
/
Drug Synergism
/
Antineoplastic Agents, Phytogenic
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2008
Type:
Article
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