Sequence analysis of a novel HLA-A * 2459 allele / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1090-1092, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-318783
ABSTRACT
This study was aimed to investigate the molecular genetics basis of a novel allele HLA-A * 2459 in Chinese population. DNA was extracted from whole blood by PEL-FREEZ DNA extraction kit. The amplification of HLA-A exons 2 - 4 of the proband was preformed by allele specific primer PCR and the amplified product was sequenced bidirectionally with primers. The sequencing results showed HLA-A alleles of the proband as A * 1101 and the novel allele. The sequences of the novel allele have been submitted to GenBank (DQ313255, DQ313256, DQ313257). After Blast HLA analysis, the novel allele showed only one nucleotide differences with HLA-A * 24020101 at nucleotide position 527 T to C in exon 3. This results in an amino acid changes from Val to Ala at codon 152. In conclusion, this allele is a novel one and has been officially named HLA-A * 2459 by the WHO Nomenclature Committee.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Blood Donors
/
Molecular Sequence Data
/
Base Sequence
/
HLA-A Antigens
/
China
/
Sequence Analysis, DNA
/
Asian People
/
Alleles
/
Genetics
Limits:
Humans
Country/Region as subject:
Asia
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2007
Type:
Article
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