Prokaryotic expression of HLA-B * 2705 heavy chain and identification for its activity / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 1028-1031, 2007.
Article
in Chinese
| WPRIM
| ID: wpr-318796
ABSTRACT
In order to investigate the expression of heavy chain of HLA-B * 2705 in prokaryotic system and identify its activity, the extra-membrane gene fragment of HLA-B * 2705 was amplified from full-length HLA-B*2705 cDNA by PCR and cloned into pGEM-T vector. After identification by sequencing, the prokaryotic expressing vector pET32a (+)-B * 2705 was constructed. The antigenic activity of expressed protein was identified by Western blot and antibody blocking reaction. The results indicated that the fused HLA-B * 2705 protein expression with high efficiency was obtained. The expressed product was more than 50% of the total bacteria protein. The antigenic activity of expressed protein was confirmed by Western blot and antibody blocking reaction. It is concluded that HLA-B * 2705 fusion protein are obtained as basis for the further studies.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
HLA-B27 Antigen
/
Classification
/
Immunoglobulin Heavy Chains
/
Allergy and Immunology
/
Escherichia coli
/
Genetic Vectors
/
Genetics
/
Metabolism
Type of study:
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2007
Type:
Article
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