Treatment of rabbit corneal with skin epidermal stem cells / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 674-677, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-318839
ABSTRACT
<p><b>OBJECTIVE</b>To construct artificial rabbit corneas with autologous skin epidermal stem cells and allogenic stromal cells in vitro and promote healing of corneal wounds.</p><p><b>METHODS</b>Skin epidermal stem cells were isolated from autologous skin samples. Keratocytes were isolated from newborn cornea biopsies. The cells were combined with acellular porcine corneal stroma scaffold to construct artificial corneas. Then the constructed artificial corneas were used to repair severe vision loss caused by complete loss of corneal epithelial stem cells.</p><p><b>RESULTS</b>Cultured skin epidermal stem cells and keratocytes were in good growth conditions. Cultured artificial corneas consisted of multiplayer epithelial cells growing on stroma equivalent consisting of stromal matrix with incorporated keratocytes. The in vitro constructed artificial corneas were histologically similar to normal rabbit corneas. Three months after transplantation, the cornea wounds were healed and the rabbit cornea became transparent.</p><p><b>CONCLUSION</b>The artificial corneas were constructed successfully in vitro and can be used to repair severe vision loss caused by complete loss of corneal epithelial stem cells.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Prostheses and Implants
/
Stem Cells
/
General Surgery
/
Wounds and Injuries
/
Eye Injuries
/
Limbus Corneae
/
Corneal Stroma
/
Cell Biology
/
Tissue Engineering
/
Epidermis
Limits:
Animals
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2005
Type:
Article
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