NS398 induced apoptosis in pancreatic carcinoma cell strain BxPC-3 through a COX-2-in dependent pathway / 中国医学科学院学报
Acta Academiae Medicinae Sinicae
;
(6): 601-605, 2005.
Article
in Chinese
| WPRIM
| ID: wpr-318855
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of the selective cyclooxygenase-2 (COX-2) inhibitor NS398 on the growth of human pancreatic tumor BxPC-3 cell strain and its possible mechanisms.</p><p><b>METHODS</b>The effect of NS398 on cell growth was assessed by 3- (4,5-dimethylthiazol-2-yl) -2, 5-diphenyl thiazolyl blue (MTT) assay. Apoptosis was determined by fluorescence-activated cell scanning (FACS) analysis and assessment of the floating cell/attached cell ratio. Caspase-3 activation was evaluated by Active Caspase-3 Apoptosis Kit with flow cytometry. Reverse transcriptase-polymerase chain reaction analysis (RT-PCR) and Western blot were used to demonstrate expression levels of COX-1, COX-2 mRNA, and protein, as well as Caspase-3 protein in pancreatic tumor BxPC-3 cell strain.</p><p><b>RESULTS</b>Selective COX-2 inhibitor NS398 significantly decreased cell viability and induced apoptosis in pancreatic tumor BxPC-3 cell strain. The protein expression of Caspase-3 was induced by high-concentration NS398. Caspase-3 activity was strongly activated by NS398.</p><p><b>CONCLUSIONS</b>Selective COX-2 inhibitor NS398 has antiproliferative and proapoptotic potential in pancreatic tumor BxPC-3 cells. Such effect is independent of COX-2, but correlates with Caspase-3 activation.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pancreatic Neoplasms
/
Pathology
/
Pharmacology
/
Sulfonamides
/
Tumor Cells, Cultured
/
Cyclooxygenase Inhibitors
/
Apoptosis
/
Cyclooxygenase 1
/
Cyclooxygenase 2
/
Caspase 3
Limits:
Humans
Language:
Chinese
Journal:
Acta Academiae Medicinae Sinicae
Year:
2005
Type:
Article
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