Effect of protein kinase C on K(V) channel in rat bronchial smooth muscle / 生理学报
Acta Physiologica Sinica
;
(6): 135-141, 2003.
Article
in Chinese
| WPRIM
| ID: wpr-318928
ABSTRACT
The effect of protein kinase C (PKC) signaling pathway on the activity of voltage-dependent delayed rectifier potassium channel (K(V)) and the expression of K(V) isoform K(V)1.5 in rat bronchial smooth cells (BSMCs) were investigated with whole-cell patch clamp, Western-blot and RT-PCR techniques. The results showed (1) phorbol 12-myristate 13-acetate (PMA), a PKC activator, caused a significant inhibition of K(V) channel currents in rat BSMCs. The inhibition was partly abolished by Ro31-8220, a PKC inhibitor. (2) PMA caused a significant suppression of the expression of K(V)1.5 mRNA and protein in rat BSMCs. These effects were attenuated by Ro31-8220. The results suggest that in rat BSMCs PKC activation inhibits K(V) currents and down-regulates the expression of K(V)1.5.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Physiology
/
Protein Kinase C
/
Bronchi
/
RNA, Messenger
/
Tetradecanoylphorbol Acetate
/
Cells, Cultured
/
Rats, Sprague-Dawley
/
Patch-Clamp Techniques
/
Cell Biology
Limits:
Animals
Language:
Chinese
Journal:
Acta Physiologica Sinica
Year:
2003
Type:
Article
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