Role of unsaturated fatty acids in the enhancement of muscarinic current by hyposmotic membrane stretch in guinea pig smooth muscle cells / 生理学报
Acta Physiologica Sinica
;
(6): 96-100, 2003.
Article
in English
| WPRIM
| ID: wpr-318935
ABSTRACT
To investigate the function of exogenous unsaturated fatty acids in hyposmotic membrane stretch enhancement of muscarinic current (ICCh) in antral circular smooth muscle cells of guinea pig, we recorded the membrane current with the conventional whole cell patch-clamp technique. I(CCh) elicited by 50 micromol/L carbachol (CCh) at the holding potential of 20 mV under isosmotic condition was taken as control. Hyposmotic membrane stretch increased I(CCh) to 226.0+/-21.0%. When the cells were pretreated with 5 micromol/L arachidonic acid (AA), linoleic acid (LA) or oleic acid (OA), I(CCh)was inhibited to 3.8+/-0.6%, 35.2+/-0.8% and 66.6+/-0.6% respectively. Hyposmotic membrane stretch increased I(CCh) to 106.0+/-2.5%, 173.2+/-6.8% and 222.1+/-11.0% of the control respectively. Five micromol/L AA inhibited hyposmotic membrane stretch-enhanced I(CCh) by 51.2+/-3.8%, while the control I(CCh) under isosmotic condition was inhibited by 96.2+/-1.6%. The results suggest that unsaturated fatty acids inhibited I(CCh) and the inhibitory effect is more significant when the unsaturation degree is increased. However, the unsaturated fatty acids are not involved in the increase of I(CCh) induced by hyposmotic membrane stretch.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osmotic Pressure
/
Pharmacology
/
Physiology
/
Pyloric Antrum
/
Sodium Chloride
/
Receptors, Muscarinic
/
Patch-Clamp Techniques
/
Cell Biology
/
Myocytes, Smooth Muscle
/
Fatty Acids, Unsaturated
Limits:
Animals
Language:
English
Journal:
Acta Physiologica Sinica
Year:
2003
Type:
Article
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