Calbindin D-28k-containing neurons receiving visceral and somatic nociceptive information in interstitial nucleus of the spinal trigeminal tract project to the parabrachial nuclei in the rat / 生理学报

ABSTRACT

The calbindin D-28k (CB)-containing neurons in the interstitial nucleus of the spinal trigeminal tract (INV) that receive visceral and orofacial somatic nociceptive information and emanate projections to the parabrachial nuclei (PB) were investigated by the triple-labeled methods of fluorogold (FG) retrograde tracing combined with Fos and CB proteins immunofluorescence histochemistry in the rat. The results showed (1) in the perioral stimulation group, a large number of FG-retrograde labeled and Fos-immunoreactive neurons were found in the paratrigeminal nucleus (PaV) and the dorsal paramarginal nucleus (PaMd) of the INV ipsilateral to FG and formalin injection made to the PB and lips, respectively, while a lot of CB-immunoreactive neurons were distributed in the INV bilaterally; (2) a majority of the FG-retrograde labeled neurons (77.3%) were double-labeled with CB, and 40.7% of them were double-labeled with Fos; about 38.5% of FG/CB double-labeled neurons were FG/CB/Fos triple-labeled in the INV; and (3) in the upper alimentary tract stimulation group, the distribution and the numbers of FG-retrograde labeled, CB-immunoreactive neurons and FG/CB double-labeled neurons in the INV were similar to those of the perioral stimulation group as described above, except that the Fos immunoreactive neurons were distributed in the INV bilaterally, approximately 41.9% of the FG-retrograde labeled neurons were FG/Fos double-labeled, and over half (52.0%) of those double-labeled neurons were FG/CB/Fos triple-labeled. The results indicate that a part of CB-containing neurons in the INV receive orofacial somatic and visceral nociceptive information and that these neurons sent projections directly to the PB. The CB-containing neurons might play an important role in the transmission of the peripheral nociceptive information from INV to PB.