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Inhibitory effect of adrenomedullin on L-type calcium currents in guinea-pig ventricular myocytes / 生理学报
Acta Physiologica Sinica ; (6): 479-484, 2002.
Article in English | WPRIM | ID: wpr-318964
ABSTRACT
The effects of adrenomedullin (ADM) on the L-type calcium currents (I(Ca,L)) and the mechanism of the signal transduction process were studied. Enzymatically isolated guinea-pig ventricular myocytes were used to measure ICa,L with whole-cell patch-clamp techniques. ADM at the concentrations of 1-100 nmol/L decreased ICa,L in a dose-dependent manner (P<0.05). ADM22-52) (100 nmol/L), a specific ADM-receptor antagonist, completely abolished the ADM-induced inhibition of ICa,L. Pretreatment of the cells with H-89 (10 micromol/L), a specific PKA inhibitor, did not attenuate the effects of ADM. Intracellular application of 10 micromol/L PKC19-36), a specific PKC inhibitor, prevented the ADM-induced inhibition of the ICa,L, while the specific PKC activator PMA could mimic the effects of ADM on the ICa,L. PMA (1 micromol/L) decreased the ICa,L by 32.26+/-4.20%(P<0.05). These findings indicate that ADM can inhibit the ICa,L in guinea-pig ventricular myocytes, and the inhibition is mediated by the specific ADM-receptor and an activation of protein kinase C.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Protein Kinase C / Patch-Clamp Techniques / Calcium Channels, L-Type / Cell Biology / Myocytes, Cardiac / Adrenomedullin / Guinea Pigs / Heart Ventricles / Metabolism Limits: Animals Language: English Journal: Acta Physiologica Sinica Year: 2002 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Protein Kinase C / Patch-Clamp Techniques / Calcium Channels, L-Type / Cell Biology / Myocytes, Cardiac / Adrenomedullin / Guinea Pigs / Heart Ventricles / Metabolism Limits: Animals Language: English Journal: Acta Physiologica Sinica Year: 2002 Type: Article