Colocalization of 11beta-hydroxysteroid dehydrogenase type I and glucocorticoid receptor and its significance in rat hippocampus / 生理学报
Acta Physiologica Sinica
;
(6): 473-478, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-318965
ABSTRACT
This paper was designed to observe the colocalization of 11beta-HSD1 and GR, and its significance in the rat hippocampus. Immunocytochemical dual-staining showed that not only 11beta-HSD1 but also GR immunoreactive substances were present in the cultured rat hippocampal neurons. Moreover, they were colocalized in the same hippocampal neuron. Synthetic glucocorticoid dexamethasone (DEX) up-regulated the protein expression and activity of 11beta-HSD1 in the cultured hippocampal neurons, as determined by Western blot and thin layer chromatography (TLC) respectively. The transfection of PC12 cells with the plasmid containing promoter sequence of 11beta-HSD1 gene and the reporter gene of CAT enzyme was conducted. DEX up-regulated the reporter gene expression in the system described above. The up-regulation of 11beta-HSD1 and reporter gene expression induced by DEX were both blocked by GR antagonist RU38486. Our study suggests that the colocalization of 11beta-HSD1 and GR in the hippocampus may be implicated in the up-regulation of 11beta-HSD1 expression by glucocorticoids combining to its promoter region, which in turn produces more biologically active glucocorticoids necessary for the binding of low affinity of GR.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Dexamethasone
/
Transfection
/
Receptors, Glucocorticoid
/
Mifepristone
/
Chemistry
/
Gene Expression Regulation
/
Promoter Regions, Genetic
/
PC12 Cells
/
Cell Biology
Limits:
Animals
Language:
Chinese
Journal:
Acta Physiologica Sinica
Year:
2002
Type:
Article
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