Effects of low concentration of dihydroouabain on intracellular calcium in guinea pig ventricular myocytes / 生理学报
Acta Physiologica Sinica
;
(6): 385-389, 2002.
Article
in English
| WPRIM
| ID: wpr-318980
ABSTRACT
The effects of low concentration of dihydroouabain (DHO) on intracellular calcium concentration ([Ca(2+)](i)) were investigated in guinea pig ventricular myocytes. [Ca(2+)](i) was detected by confocal microscopy and represented by fluorescent intensity. DHO (1 fmol/L~1 mmol/L) increased [Ca(2+)](i), especially at 10 pmol/L. Nisoldipine, egtazic acid, or tetrodotoxin partially inhibited the effect of 10 pmol/L DHO on [Ca(2+)](i). The effects of DHO remained in the absence of extracellular K(+) and Na(+). These results suggest that low concentration of DHO might increase [Ca(2+)](i) via the receptor-operated Ca(2+) channels, TTX-sensitive Na(+) channels or/and triggering of intracellular calcium release; Na(+)/K(+) pump and Na(+)/Ca(2+) exchange seem not involved in the effect of DHO.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Ouabain
/
Pharmacology
/
Calcium
/
Patch-Clamp Techniques
/
Cell Biology
/
Myocytes, Cardiac
/
Guinea Pigs
/
Heart Ventricles
/
Metabolism
Limits:
Animals
Language:
English
Journal:
Acta Physiologica Sinica
Year:
2002
Type:
Article
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