Cloning genes sensitive to mechanical stretch in osteoblasts through subtractive hybridization technique / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 278-280, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-319000
ABSTRACT
<p><b>OBJECTIVE</b>In this experiment, genes sensitive to mechanical stretch in osteoblast like cells were cloned through subtractive hybridization technique.</p><p><b>METHODS</b>Two dimensional mechanical stretch with deformation of 12% and frequency of 6 cycles was loaded on human osteoblastic like cell line Saos-2. Complementary deoxyribonucleic acid (cDNA) library of cells was constructed 12 h after loading, acting as tester. cDNA library of cells without loading was constructed, acting as driver. A subtractive cDNA library osteoblastic like cell stimulated with mechanical stretch was constructed through subtractive hybridization technique.</p><p><b>RESULTS</b>Of clones randomly selected from this library, fifteen genes were identified to be the differentially expressed genes. Comparing with the sequences published in GeneBank via Internet, two sequences located in chromosome 9 and 18 respectively were identified to be novel, which were named as stretch sensitive gene 1 and stretch sensitive gene 2.</p><p><b>CONCLUSION</b>It is an efficient approach to clone and study genes relative to mechanical stretch through subtractive hybridization technique.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Osteoblasts
/
Physiology
/
Stress, Mechanical
/
Gene Library
/
Cell Line
/
Cloning, Molecular
/
Gene Expression Profiling
/
Nucleic Acid Hybridization
Limits:
Humans
Language:
Chinese
Journal:
West China Journal of Stomatology
Year:
2004
Type:
Article
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