In vitro and in vivo study on proliferation of adnoid cystic carcinoma cell lines after nm23-h1. Introduction / 华西口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the effect of nm23-h1 transfection on proliferation characteristics of adnoid carcinoma cell lines in vitro and in vivo.</p><p><b>METHODS</b>In vitro ACC-M cell lines were incubated after putative anti-metastatic gene nm23-h1 was introduced into the cells with the help of G418 selective incubation base. The ACC-M cells were transplanted into 10 BALB/C nude mices subcutaneously and non-transfected cell lines were taken as control. Immunohisto chemistry and Ki67 antibody were employed to study the proliferation character of cell crawling pieces and paraffin-bedded slice, meanwhile, the solid tumor of both groups were prepared for flow cytometry(FCM).</p><p><b>RESULTS</b>Transfected cells grew slower than non-transfected cells and this trend became more obvious as passages passed on. In vitro the expression of Ki67 of transfected cells was little stronger than non-transfected cells, while the expression of Ki67 in solid slices was almost negative in both groups. Transfected cells presented slower growth than non-transfected cells in the early stage (2 weeks) and 2 weeks later there was no obvious difference in size(P > 0.05). FCM value accorded well with the result.</p><p><b>CONCLUSION</b>Introduction of nm23-h1 into the ACC-M cell lines may have transient inhibitory effects on its proliferation.</p>