Characterization of Notch gene involved in genetic regulatory networks in dental pulp stem cells / 华西口腔医学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the characterization of Notch gene involved in genetic regulatory networks in dental pulp stem cells.</p><p><b>METHODS</b>The pulp tissue was separated from mouse teeth and digested by collagenase type I. Single-cell suspensions of dental pulp were seeded into 6-well plates with alpha modification of Eagle's medium supplemented with ES cell qualified Fetal Bovine Serum. Colony-forming efficiency was assessed in 14ds culture. Transcripts for Notch were detected by reverse transcription-PCR by using total RNA isolated from cells.</p><p><b>RESULTS</b>There were clonogenic cells in dental pulp cell and the incidence of colony-forming cells derived from mouse dental pulp cells was 1.6-2.5 colonies/10(4) plate. Mouse-specific Notch mRNA expressed in colony-forming cells.</p><p><b>CONCLUSION</b>Notch mRNA expressing in colony-forming cells provided a more detailed understanding of mouse dental pulp stem cell biology.</p>