Expression of GBD gene of Streptococcus mutans glucan binding protein A in mammalian cells / 华西口腔医学杂志
West China Journal of Stomatology
;
(6): 10-12, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-319073
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the expression of recombinant plasmid pcDNA3.1/GBD of glucan binding protein of Streptococcus mutans in mammalian cells COS-7.</p><p><b>METHODS</b>Eukaryotic plasmid carrying encoding gene of GBD of Streptococcus mutans gbpA was constructed and the plasmid was introduced into COS-7 cells by Lipofectamine reagent. The transient expressed protein in COS-7 cells was detected by immunochemistry technique.</p><p><b>RESULTS</b>The positive expression was detected in plasma of the cells which were transfected with recombinant plasmid pcDNA3.1/GBD. The cells which were transfected with pcDNA3.1 were negative.</p><p><b>CONCLUSION</b>GBD can translate and express in COS-7 cells after transfected with recombinant plasmid pcDNA3.1/GBD. The expressed protein locates in the plasma and the protein is able to combine with anti-GbpA antibody. The expressed protein has the antigenicity and is a candidate gene vaccine.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Plasmids
/
Streptococcus mutans
/
Bacterial Proteins
/
Recombinant Proteins
/
Transfection
/
Carrier Proteins
/
Gene Expression
/
COS Cells
/
Vaccines, DNA
/
Dental Caries
Limits:
Animals
/
Humans
Language:
Chinese
Journal:
West China Journal of Stomatology
Year:
2004
Type:
Article
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