Proteomic identification of necrozoospermia-related proteins / 中华男科学杂志
National Journal of Andrology
;
(12): 431-435, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-319218
ABSTRACT
<p><b>OBJECTIVE</b>To compare the proteomic differences between normal and necrozoospermic human spermatozoa.</p><p><b>METHODS</b>We isolated proteins from 30 ejaculates of 10 healthy donors and 3 specimens of a necrozoospermia patient by two-dimensional gel electrophoresis (2-DE), analyzed their 2-DE maps and identified some differentially expressed proteins by mass spectrometry.</p><p><b>RESULTS</b>A total of (905 +/- 57) and (792 +/- 28) proteins were isolated from the spermatozoal maps of the normal and necrozoospermic men, respectively, and 178 found to be differentially expressed in that of the necrozoospermia patient. The 6 missing protein spots in the necrozoospermic map were identified as sperm protein (human, accession No. 060904), zinc finger protein 174 (AW-1), F-actin capping protein alpha-3 subunit, testis-specific inhibitor of apoptosis, death domain receptor 3 soluble form (fragment) and peptide similar to the activator of CREM in the testis.</p><p><b>CONCLUSION</b>Six missing protein spots were identified in the necrozoospermic spermatozoal map which may be associated with the development of necrozoospermia.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oligospermia
/
Pathology
/
Mass Spectrometry
/
Spermatozoa
/
Peptide Mapping
/
Electrophoresis, Gel, Two-Dimensional
/
Proteome
/
Proteomics
/
Metabolism
/
Methods
Limits:
Humans
/
Male
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2008
Type:
Article
Similar
MEDLINE
...
LILACS
LIS