PI-3K and p38MAPK pathways upregulate the epidermal growth factor induced cyclooxygenase-2 expression in PC-3 cells / 中华男科学杂志
National Journal of Andrology
; (12): 220-223, 2008.
Article
in Zh
| WPRIM
| ID: wpr-319242
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of PI-3K and p38MAPK signal pathways on the cyclooxygenase-2 (COX-2) expression induced by the epidermal growth factor (EGF) in PC-3 cells.</p><p><b>METHODS</b>PC-3 cell proliferation was detected by methylthiazolyl tetrazolium (MTT) assay after stimulated by EGF (0 microg/L), EGF (10 microg/L), EGF (10 microg/L) + LY294002 (20 micromol/L) and EGF (10 microg/L) + SC203580 (20 micromol/L), and so was the COX-2 expression in the PC-3 cells by RT-PCR and Western blot assay after stimulated the same way for 24 hours. ELISA was used to determine the changes of PGE2 in the culture medium.</p><p><b>RESULTS</b>LY294002 and SC203580 signficantly inhibited PC-3 cell proliferation (P < 0.05), COX-2 expression and PGE2 production after EGF stimulation (P < 0.05).</p><p><b>CONCLUSION</b>EGF can stimulate PC-3 cells into proliferation and induce COX-2 mRNA and the upregulation of its protein expression, while LY294002 and SC203580 can inhibit EGF from the above effects on PC-3 cells.</p>
Full text:
1
Index:
WPRIM
Main subject:
Pharmacology
/
Enzyme-Linked Immunosorbent Assay
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Dinoprostone
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Signal Transduction
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Gene Expression
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Blotting, Western
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Phosphatidylinositol 3-Kinases
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Reverse Transcriptase Polymerase Chain Reaction
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Cell Line, Tumor
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P38 Mitogen-Activated Protein Kinases
Limits:
Humans
/
Male
Language:
Zh
Journal:
National Journal of Andrology
Year:
2008
Type:
Article