Effects of free fatty acids on insulin signaling proteins in rat islet cells / 中国应用生理学杂志
Chinese Journal of Applied Physiology
;
(6): 283-286, 2002.
Article
in Chinese
| WPRIM
| ID: wpr-319306
ABSTRACT
<p><b>AIM</b>To investigate whether free fatty acid impair insulin signaling proteins to inhibit insulin action in rat islet cells.</p><p><b>METHODS</b>After rat islet cells were incubated with palmitate (0.25 mmol/L) or oleate (0.125 mmol/L) for 12 hours, 24 hours and 36 hours, western bolt was used to assess the protein abundance of cPKCalpha, Grb2 and FERK2.</p><p><b>RESULTS</b>The protein content of cPKCalpha were significantly upregulated and the protein abundance of Grb2 and ERK2 was decreased comparing with control in rat islet cells after incubated with free fatty acids.</p><p><b>CONCLUSIONS</b>Free fatty acids may inhibit insulin activity in rat islet cells through up-regulating the expression of cPKCalpha or down-regulating the expression of Grb2 and ERK2.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Blood
/
Signal Transduction
/
Cells, Cultured
/
Islets of Langerhans
/
Rats, Sprague-Dawley
/
Mitogen-Activated Protein Kinase 1
/
Protein Kinase C-alpha
/
GRB2 Adaptor Protein
/
Fatty Acids, Nonesterified
/
Insulin
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2002
Type:
Article
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