Comparison of pharmacological characteristics of the endothelial target for acetylcholine between big artery and small artery / 中国应用生理学杂志

ABSTRACT

<p><b>AIM</b>To compare the differences of pharmacological characteristics of the endothelial target for acetylcholine (ETA) between rat aorta and tail artery.</p><p><b>METHODS</b>Differences in the endothelium-dependent relaxation induced by acetylcholine (ACh 10(-8) - 10(-4) mol/L) were studied using isolated rat tail artery helical strips and aortic rings, so that the pharmacological characteristics of ETA in small artery can be observed.</p><p><b>RESULTS</b>ACh-induced endothelium-dependent relaxation was observed both in rat tail artery strips and in aortic rings precontracted with potassium chloride (60 mmol/L) in a concentration-dependent manner. In tail artery this effect was partially blocked by L-N(omega)-Nitro-arginine methyl ester (L-NAME 10(-4) mol/L) or methylene blue (MB 10(-5) mol/L), together with indomethacin (Indo 10(-4) mol/L), but in aorta it was completely blocked by L-NAME or MB.</p><p><b>CONCLUSION</b>It is different of the pharmacological characteristics of ETA between big artery and small artery. A non-NO and non-PGI2 relaxing factor, together with nitric oxide (NO) and prostacyclin (PGI2), mediates endothelium-dependent vasorelaxation induced by ACh in small artery, but NO may be the principal endothelial vasodilator substance in big artery.</p>