Activation of PACAP receptor could protect cultured hippocampal neurons against beta amyloid peptide induced neurotoxicity / 中国应用生理学杂志

ABSTRACT

<p><b>AIM</b>To observe the protective role of pituitary adenylate cyclase activating polypeptide (PACAP) on hippocampal neuronal apoptosis induced by beta amyloid peptide in the culture.</p><p><b>METHODS</b>Hippocampal neurons were isolated from 1d old SD rat and neuronal survival and apoptosis were measured by MTT assay and DNA ladder.</p><p><b>RESULTS</b>25 micromol/L Abeta could induce neuron apoptosis while co-treatment with PACAP could increase the survival of hippocampal neurons. The antagonist of PACAP receptor, P6-27, could reverse the effect of PACAP.</p><p><b>CONCLUSION</b>PACAP could protects cultured neurons from the neurotoxicity of Abeta through the activation of PACAP receptor and may have a bright use in treatment of neurodegenerative disease.</p>