High concentration uric acid regulates endothelial function via miR-155 / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1141-1145, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-319460
ABSTRACT
<p><b>OBJECTIVE</b>To investigate high-concentration uric acid-induced endothelial dysfunction mediated by miR-155.</p><p><b>METHODS</b>Human umbilical vein endothelial cells (HUVECs) were incubated with 600 µmol/L uric acid for 24 and 48 h, and eNOS expression and NO content in the cell culture were measured. The target genes of miR-155 were predicted using on-line analysis software and validated by dual-luciferase system. Real-time PCR was used to detect the expression of miR-155 in endothelial cells incubated with high-concentration uric acid. The effect of miR-155 on endothelial dysfunction was assessed by transfection of its inhibitor into HUVECs.</p><p><b>RESULTS</b>The expression of eNOS and NO secretion decreased obviously in HUVECs incubated with 600 µmol/L uric acid. MicroRNA on-line analysis software and dual luciferase reporter experiments suggested that the level of eNOS translation was directly regulated by miR-155. The expression of miR-155 in endothelial cells was upregulated after stimulation with high-concentration uric acid, and was inhibited by transfection of miR-155 inhibitor. Expression of eNOS and secretion of NO were elevated in endothelial cells transfected with miR-155 inhibitor after incubation with high-concentration uric acid.</p><p><b>CONCLUSION</b>High-concentration uric acid can down-regulate eNOS expression via miR-155 to induce endothelial dysfunction.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Uric Acid
/
Blood
/
Down-Regulation
/
Cells, Cultured
/
Cell Biology
/
Hyperuricemia
/
MicroRNAs
/
Nitric Oxide Synthase Type III
/
Human Umbilical Vein Endothelial Cells
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2013
Type:
Article
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