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β-amyloid protein up-regulates the expression of the receptor for advanced glycation end products by increasing ROS production / 南方医科大学学报
Article in Zh | WPRIM | ID: wpr-319462
Responsible library: WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the mechanism of β-amyloid protein (Aβ) in regulating the expression of the receptor for advanced glycation end products (RAGE).</p><p><b>METHODS</b>Aβ1-40 was injected into the bilateral hippocampus of rats, and 3 weeks later, the levels of reactive oxygen species (ROS) production were detected by flow cytometry. The expressions of RAGE, reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidases (gp9l(phox) and p47(phox)), nuclear factor-κB (NF-κB), and inhibitor of κB (IκB) were measured by Western blotting.</p><p><b>RESULTS</b>Injection of Aβ1-40 caused a significant increase in the expressions of RAGE, gp9l(phox), p47(phox), phospho-p47(phox), phospho-IκBα, NF-κB and phospho-NF-κB in rat hippocampus but decreased the level of IκBα. Aβ1-40 injection also resulted in a significantly increased content of ROS in the hippocampus of the rats.</p><p><b>CONCLUSION</b>Aβ up-regulates the expression of RAGE in rat hippocampus via NADPH/ ROS/NF-κB signaling pathway.</p>
Subject(s)
Full text: 1 Index: WPRIM Main subject: Peptide Fragments / Membrane Glycoproteins / Receptors, Immunologic / Signal Transduction / Up-Regulation / Amyloid beta-Peptides / NF-kappa B / Reactive Oxygen Species / Rats, Sprague-Dawley / Oxidative Stress Limits: Animals Language: Zh Journal: Journal of Southern Medical University Year: 2013 Type: Article
Full text: 1 Index: WPRIM Main subject: Peptide Fragments / Membrane Glycoproteins / Receptors, Immunologic / Signal Transduction / Up-Regulation / Amyloid beta-Peptides / NF-kappa B / Reactive Oxygen Species / Rats, Sprague-Dawley / Oxidative Stress Limits: Animals Language: Zh Journal: Journal of Southern Medical University Year: 2013 Type: Article