Effects of gastrin on rat intestinal epithelial 1,25(OH)2D3-membrane associated rapid response steroid binding protein / 南方医科大学学报
Journal of Southern Medical University
;
(12): 990-993, 2013.
Article
in Chinese
| WPRIM
| ID: wpr-319494
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effects of gastrin on the expression of 1,25(OH)2D3-membrane associated rapid response steroid (1,25D3-MARRS) binding protein in rat intestinal epithelium.</p><p><b>METHODS</b>SD rats received intraperitoneal injections of gastrin, omeprazole or physiological saline. The protein expression of 1,25D3-MARRS binding protein in SD rat intestinal was determined with Western blotting and immunohistochemistry, and its mRNA levels determined by RT-PCR. The serum calcium and phosphate levels in the rats were also detected.</p><p><b>RESULTS</b>Immunohistochemistry showed that 1,25D3-MARRS binding protein was expressed mainly in the nuclei, cytoplasm and membrane of the intestinal epithelial cells. Both the protein and mRNA expression levels of 1,25D3-MARRS binding protein were up-regulated after treatments with gastrin and omeprazole (P<0.05), but the serum calcium and phosphate concentrations showed no obvious increase.</p><p><b>CONCLUSION</b>1,25D3-MARRS binding protein, which is widely expressed with versatile functionalities, is regulated by gastrin and shows high potentials in the study of gastrointestinal diseases.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Calcitriol
/
Gastrins
/
Rats, Sprague-Dawley
/
Protein Disulfide-Isomerases
/
Cell Biology
/
Epithelial Cells
/
Intestines
/
Metabolism
Limits:
Animals
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2013
Type:
Article
Similar
MEDLINE
...
LILACS
LIS