Expressions of chemokine CXCL12 and its receptor CXCR4 in human sperm / 中华男科学杂志
National Journal of Andrology
;
(12): 225-228, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-319515
ABSTRACT
<p><b>OBJECTIVE</b>To explore the expression patterns of the chemokine CXCL12 and its receptor CXCR4 in human sperm.</p><p><b>METHODS</b>We collected semen samples from 10 fertile men, performed density gradient centrifugation, and then determined the expressions of both CXCL12 and CXCR4 in the sperm by RT-PCR and immunofluorescence staining.</p><p><b>RESULTS</b>RT-PCR revealed the mRNA expressions of CXCL12 (0.641 +/- 0.180) and CXCR4 (0.464 +/- 0.100) in the sperm. However, only CXCR4 rather than CXCL12 was expressed at the protein level, and the positive staining for CXCR4 was observed mainly in the posterior part of the acrosome.</p><p><b>CONCLUSION</b>CXCL12 and CXCR4 are involved as important molecules in regulating the function of human sperm.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Spermatozoa
/
Acrosome
/
Signal Transduction
/
Centrifugation, Density Gradient
/
Receptors, CXCR4
/
Chemokine CXCL12
/
Metabolism
Limits:
Humans
/
Male
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2015
Type:
Article
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