Effect of sodium houttuyfonate in enhancing imipenem's activity against Pseudomonas aeruginosa biofilms / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 296-299, 2014.
Article
in Chinese
| WPRIM
| ID: wpr-319611
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the resistant effect of houttuyfonate sodium (SH) combined with imipenem (IMP) against Pseudomonas aeruginosa (Pa) biofilms.</p><p><b>METHOD</b>The two-fold dilution method was used to examine the minimum inhibitory concentration (MIC) of the tested drug. The crystal violet staining was applied to detect the effect of the combination of 1/2MIC, 1MIC, 2MIC of SH, single IMP, 1/2MIC of SH and IMP of various concentrations on the clearance rate of adherent bacteria, growth of biofilms and alginate production. Fluorescein diacetate (FDA)-propidium iodide (PI) doubling staining assay was employed to observe the bacterial viability and morphological changes after membrane dispersion of each drug group.</p><p><b>RESULT</b>Sodium houttuyfonate could enhance the effect of IMP against pseudomonas aeruginosa biofilms. Particularly, the combination group with the concentration of 2MIC showed the highest effect, with P < 0.001 compared with the negative control group. The above results were proved by the bacterial viability and biofilm morphology under fluorescence microscope.</p><p><b>CONCLUSION</b>After being combined with imipenem, sodium houttuyfonate shows a higher effect against biofilms. It is expected that the combination of the two drugs could improve the clinical efficacy of associated infections.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Physiology
/
Pseudomonas aeruginosa
/
Sulfites
/
Microbial Sensitivity Tests
/
Imipenem
/
Biofilms
/
Drug Synergism
/
Alkanes
/
Microbial Viability
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2014
Type:
Article
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