Comparative study on pro-inflammatory toxicity of Pinellia pedatiecta before and after being processed with alum / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 3893-3897, 2013.
Article
in Zh
| WPRIM
| ID: wpr-319683
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the pro-inflammatory toxicity of Pinellia pedatiecta, as well as the alum processing method on its pro-inflammatory effect.</p><p><b>METHOD</b>Raphide and agglutinin (PPA) proteins were isolated from fresh P. pedatiecta. The overall animal and cellular level models were applied to investigate the pro-inflammatory effect of raphide and PPA in P. pedatiecta, as well as the impact of the alum processing method on the pro-inflammatory effect, with inflammatory mediators as the index.</p><p><b>RESULT</b>Intraperitoneal injection with P. pedatiecta raphide suspension could significantly increase the content of inflammatory mediators PGE2 and NO. After the alum processing method was adopted, fresh P. pedatiecta and raphide-induced PGE2 and NO release significantly reduced. The stimulation of mice macrophages with P. pedatiecta agglutinin protein could cause the content of dose-dependent inflammatory mediators TNF-alpha and IL-6. After the alum processing method was adopted, PGE2 content in P. pedatiecta agglutinin protein-induced mice peritoneal exudate notably decreased.</p><p><b>CONCLUSION</b>The irritation and toxicity of P. pedatiecta were inflammatory responses in organisms. Its raphide and agglutinin proteins were toxic components, both could cause significant the release of inflammatory medium. The alum processing method could help significantly reduce the pro-inflammatory toxicity of P. pedatiecta.</p>
Full text:
1
Index:
WPRIM
Main subject:
Drugs, Chinese Herbal
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Chemistry
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Interleukin-6
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Tumor Necrosis Factor-alpha
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Inflammation Mediators
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Pinellia
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Drug Compounding
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Allergy and Immunology
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Toxicity
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Alum Compounds
Type of study:
Prognostic_studies
Limits:
Animals
Language:
Zh
Journal:
China Journal of Chinese Materia Medica
Year:
2013
Type:
Article