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Overexpression of Tiam1 gene and its relationship with invasive and metastatic ability of nasopharyngeal carcinoma / 中华病理学杂志
Chinese Journal of Pathology ; (12): 268-272, 2009.
Article in Chinese | WPRIM | ID: wpr-319712
ABSTRACT
<p><b>OBJECTIVE</b>To explore biological aspects of Tiam1 gene expression in nasopharyngeal carcinoma cells.</p><p><b>METHODS</b>Tiam1/C1199HA expression plasmids were transfected into nasopharyngeal carcinoma cells of C666-1 and CNE1 by lipofectamine2000. RT-PCR, real-time PCR and Western blot Analyses were performed to evaluate the expression of Tiam1 mRNA and protein levels, respectively. In vitro cell adhesion, wound healing and matrigel invasion assays were used to study the biological impact of Tiam1 on cell adhesion, mobility and invasion.</p><p><b>RESULTS</b>Tiam1 over expression significantly increased the abilities of adhesion, migratory and invasion of C666-1 and CNE1 cells, comparing with that of the control untransfected cells (P < 0.05).</p><p><b>CONCLUSION</b>Tiam1 expression correlates with the invasion and metastasis of nasopharyngeal carcinoma cells.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Physiology / Plasmids / RNA, Messenger / Gene Expression Regulation, Neoplastic / Cell Adhesion / Cell Movement / Nasopharyngeal Neoplasms / Guanine Nucleotide Exchange Factors / Cell Line, Tumor Limits: Humans Language: Chinese Journal: Chinese Journal of Pathology Year: 2009 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pathology / Physiology / Plasmids / RNA, Messenger / Gene Expression Regulation, Neoplastic / Cell Adhesion / Cell Movement / Nasopharyngeal Neoplasms / Guanine Nucleotide Exchange Factors / Cell Line, Tumor Limits: Humans Language: Chinese Journal: Chinese Journal of Pathology Year: 2009 Type: Article